4FA0
Crystal structure of human AdPLA to 2.65 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-01-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 59.991, 59.991, 74.006 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.140 - 2.650 |
| R-factor | 0.2079 |
| Rwork | 0.206 |
| R-free | 0.24710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2kyt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.3.0) |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.954 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.052 | 0.437 |
| Number of reflections | 7196 | |
| <I/σ(I)> | 14.6 | 2.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 293 | 25% PEG 1500, 100mM MIB buffer, pH 5.0, vapor diffusion, temperature 293K |
