4F9U
Structure of glycosylated glutaminyl cyclase from Drosophila melanogaster
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-10 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.665, 56.964, 95.669 |
Unit cell angles | 90.00, 108.16, 90.00 |
Refinement procedure
Resolution | 90.900 - 1.800 |
R-factor | 0.1706 |
Rwork | 0.170 |
R-free | 0.22480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3si2 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.488 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.902 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.079 | 0.514 |
Number of reflections | 57047 | |
<I/σ(I)> | 10.6 | 2.2 |
Completeness [%] | 99.0 | 97.8 |
Redundancy | 4.2 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 10% PEG35000, 2 mM PQ50, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |