4F9T

Ribosomal protein L1 from Thermus thermophilus with substitution Thr217Ala

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-05-02
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.072
Spacegroup name	P 21 21 2
Unit cell lengths	80.940, 62.120, 43.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.460
R-factor	0.16561
Rwork	0.164
R-free	0.20246
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.428
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.600
High resolution limit [Å]	1.460	1.460
Rmerge	0.037	0.139
Number of reflections	38090
<I/σ(I)>	25.2	9.4
Completeness [%]	97.8	94.1
Redundancy	7	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10	295	15 MG/ML L1 50 MM GLYCINE,INITIALLY AT PH 10 5% (V/V)METHANE PENTANEDIOL 1.2 M AMMONIUM SULFATE EQUILIBRATED AGAINST 2.4 M AMMONIUM SULFATE 7% (V/V) METHANE PENTANEDIOL , VAPOR DIFFUSION, HANGING DROP, temperature 295K