4F91
Brr2 Helicase Region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-23 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.1270 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.260, 150.500, 141.960 |
| Unit cell angles | 90.00, 120.21, 90.00 |
Refinement procedure
| Resolution | 48.394 - 2.697 |
| R-factor | 0.2261 |
| Rwork | 0.224 |
| R-free | 0.27160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.329 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.840 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.080 | |
| Number of reflections | 140336 | |
| <I/σ(I)> | 1.6 | |
| Completeness [%] | 98.7 | 93.9 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 0.1 M sodium citrate, 1.2 M sodium Malonate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
