4F8Z
Carboxypeptidase T with Boc-Leu
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-13 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 157.914, 157.914, 104.068 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.000 - 1.380 |
| R-factor | 0.13986 |
| Rwork | 0.139 |
| R-free | 0.15190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.194 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.120 |
| Number of reflections | 286940 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | COUNTER-DIFFUSION. protein solution: 250 mM NaCl, 1 mM CaCl2, 10 mM Mes/NaOH, pH 6.0. precipitant solution: 1.4 M (NH4)2SO4, 5% MPD, 50 mM, Mes/NaOH, pH 6.0, 2.4 M Boc-L-Leu |
