4F8T

Crystal Structure of the Human BTN3A3 Ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 2 21
Unit cell lengths	39.841, 78.430, 98.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.964 - 2.382
R-factor	0.2223
Rwork	0.221
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f80
RMSD bond length	0.011
RMSD bond angle	1.163
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.420
High resolution limit [Å]	2.380	6.460	2.380
Number of reflections	12256
Completeness [%]	94.7	99.3	66.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Sitting drop	4	298	0.1M Citric acid pH4.0, 0.8M ammonium sulfate, 0.15M Sodium Fluoride, Sitting drop, temperature 298K