4F8R

Bacillus DNA Polymerase I Large Fragment complex 7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Detector technology	CCD
Collection date	2011-11-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	93.670, 109.370, 150.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.982 - 1.640
R-factor	0.1821
Rwork	0.181
R-free	0.20340
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2HVI WITH INSERTION SITE BASE PAIR METAL IONS AND PROTEIN RESIDUES 681-721 DELETED
RMSD bond length	0.011
RMSD bond angle	1.384
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (dev_1026)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.740
High resolution limit [Å]	1.640	4.900	1.640
Rmerge	0.038	0.021	0.476
Number of reflections	172238	7324	30352
<I/σ(I)>	25.19	63.27	3.98
Completeness [%]	91.2	97.5	99.9
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	290	48%-54% Saturated Ammonium Sulfate, 2.5% MPD, 10mM MgSO4, 100mM MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K