4F7K
Crystal structure of Lac15 from a marine microbial metagenome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-01 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.410, 91.360, 86.150 |
| Unit cell angles | 90.00, 112.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.21687 |
| Rwork | 0.214 |
| R-free | 0.26534 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yxw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.461 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.083 | |
| Number of reflections | 41974 | |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 98.4 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M MES, 18% PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
