4F7B
Structure of the lysosomal domain of limp-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-24 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.772, 115.984, 145.084 |
| Unit cell angles | 90.00, 96.38, 90.00 |
Refinement procedure
| Resolution | 34.640 - 3.000 |
| R-factor | 0.1997 |
| Rwork | 0.198 |
| R-free | 0.23180 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.900 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.100 |
| Rmerge | 0.105 | 0.470 |
| Number of reflections | 67235 | |
| <I/σ(I)> | 13.34 | 2.86 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.98 | 5.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 28% PEG 1500, 0.2M Sodium Chloride and Sodium Cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
