4F6M
Crystal structure of Kaiso zinc finger DNA binding domain in complex with Kaiso binding site DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.2819 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 44.760, 185.180, 112.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.690 - 2.400 |
R-factor | 0.22306 |
Rwork | 0.222 |
R-free | 0.23986 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.838 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.103 | 0.515 |
Number of reflections | 18774 | |
<I/σ(I)> | 11.4 | 1.8 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 4.67 | 4.64 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2M sodium nitrate, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 294K |