4F62

Crystal structure of a putative farnesyl-diphosphate synthase from Marinomonas sp. MED121 (Target EFI-501980)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 32 2 1
Unit cell lengths	96.491, 96.491, 156.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.059 - 2.101
R-factor	0.1782
Rwork	0.177
R-free	0.20290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lsn
RMSD bond length	0.007
RMSD bond angle	1.012
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.076	0.034	0.751
Number of reflections	49760
<I/σ(I)>	10.3
Completeness [%]	100.0	99.4	100
Redundancy	11.8	11.4	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	protein: 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 10% glycerol, 1 mM DTT, 5 mM magnesium chloride, reservoir: 0.1 M sodium chloride, 0.1 M HEPES, pH 7.5, 1.6 M ammonium sulfate, cryoprotectant: reservoir + 20% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K