4F61
Tubulin:Stathmin-like domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-11-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 639.740, 66.100, 128.130 |
| Unit cell angles | 90.00, 92.02, 90.00 |
Refinement procedure
| Resolution | 49.040 - 4.170 |
| R-factor | 0.2421 |
| Rwork | 0.241 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ryc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.280 |
| High resolution limit [Å] | 4.170 | 4.170 |
| Number of reflections | 40336 | |
| <I/σ(I)> | 8.2 | 1.65 |
| Completeness [%] | 98.6 | 90.8 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | PEG, NACL, PIPES BUFFER, PH 6.80, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
