4F5X
Location of the dsRNA-dependent polymerase, VP1, in rotavirus particles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 740.750, 1198.070, 1345.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 5.000 |
| Rwork | 0.293 |
| R-free | 0.29580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3KZ4 AND 2R7S |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 223.000 |
| High resolution limit [Å] | 3.800 |
| Number of reflections | 1823596 |
| Completeness [%] | 20.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
