4F4D

F337R variant of human ferrochelatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	210
Detector technology	IMAGE PLATE
Collection date	2008-02-10
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	88.210, 93.511, 111.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 1.800
Rwork	0.177
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.124
RMSD bond angle	2.651
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Rmerge	0.089
Number of reflections	165467
<I/σ(I)>	40.2
Completeness [%]	99.8
Redundancy	16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1 M BIS-TRIS, 0.05 M AMMONIUM ACETATE, 20% PENTAERYTHRITOL ETHOXYLATE (15/4 EO/OH) , PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K