4F44
Neurotrophin p75NTR intracellular domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-09 |
| Detector | RIGAKU SATURN 70 |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 32 |
| Unit cell lengths | 53.372, 53.372, 73.486 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.560 - 2.400 |
| R-factor | 0.23024 |
| Rwork | 0.228 |
| R-free | 0.28001 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ngr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.111 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 8699 | |
| Completeness [%] | 84.0 | 84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1M Bis-Tris propane, 1.4M sodium malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
