4F3M
Crystal structure of CRISPR-associated protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9770 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 86.509, 46.687, 129.030 |
Unit cell angles | 90.00, 104.01, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.710 |
R-factor | 0.1565 |
Rwork | 0.152 |
R-free | 0.23010 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.685 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 50063 | |
Completeness [%] | 91.8 | 76.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.5 | 0.1 M MES, 0.8 M Ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.5K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.5 | 0.1 M MES, 0.8 M Ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.5K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.5 | 0.1 M MES, 0.8 M Ammonium sulfate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.5K |