4F3J

Crystal Structure of Trimeric gC1q Domain of Human C1QTNF5 associated with Late-onset Retinal Macular Degeneration

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	93
Detector technology	PIXEL
Collection date	2012-04-02
Detector	PSI PILATUS 6M
Wavelength(s)	0.97920
Spacegroup name	H 3
Unit cell lengths	46.621, 46.621, 138.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.277 - 1.337
R-factor	0.1378
Rwork	0.136
R-free	0.16490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.373
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.277	46.277	1.410
High resolution limit [Å]	1.337	4.220	1.337
Rmerge		0.082	0.324
Total number of observations		3957	8392
Number of reflections	25129
<I/σ(I)>	20.4	6.1	2.2
Completeness [%]	98.3	100	89.1
Redundancy	4.8	4.9	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	0.1 M Sodium Acetate pH 5.0, 2 M NaCl, 10% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K