4F37
Structure of the tethered N-terminus of Alzheimer's disease A peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.94721 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.642, 82.836, 89.196 |
| Unit cell angles | 90.05, 92.51, 90.00 |
Refinement procedure
| Resolution | 44.460 - 2.570 |
| R-factor | 0.22564 |
| Rwork | 0.223 |
| R-free | 0.26885 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.587 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.560 | 2.640 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.170 | |
| Number of reflections | 28836 | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 89.1 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Sodium malonate, 20% (w/v) PEG 3350, 0.1M Bis-tris propane pH 8.5, 0.2M 1-methylimidazolium formate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
