4F2Z
Crystal structure of RPE65 in a lipid environment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0750 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.799, 100.234, 277.588 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.400 - 3.000 |
R-factor | 0.22561 |
Rwork | 0.224 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fsn |
RMSD bond length | 0.006 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.190 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.132 | 0.817 |
Number of reflections | 31980 | |
<I/σ(I)> | 13.3 | 2.43 |
Completeness [%] | 99.7 | 99 |
Redundancy | 6.8 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 281 | 30% PEG200, 3% PEG3350, pH 7.0, grown directly from solubilized RPE microsomes, VAPOR DIFFUSION, HANGING DROP, temperature 281K |