4F2M
Crystal structure of a TGEV coronavirus Spike fragment in complex with the TGEV neutralizing monoclonal antibody 1AF10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2007-02-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.770, 106.050, 103.520 |
| Unit cell angles | 90.00, 95.13, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.000 |
| R-factor | 0.2178 |
| Rwork | 0.216 |
| R-free | 0.25070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.207 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.074 | 0.354 |
| Number of reflections | 33913 | |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 94.7 | 95.3 |
| Redundancy | 5.6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 16% PEG4000, 0.2 M sodium acetate, 0.1 M 1,2,3-octanetriol isomer T, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
