4F28
The Crystal Structure of a Human MitoNEET mutant with Met 62 Replaced by a Gly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-16 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.330, 56.600, 59.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.870 - 1.550 |
R-factor | 0.2177 |
Rwork | 0.216 |
R-free | 0.24880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.870 | 1.610 | |
High resolution limit [Å] | 1.550 | 3.330 | 1.550 |
Rmerge | 0.047 | 0.034 | 0.991 |
Number of reflections | 20457 | 2223 | 1899 |
<I/σ(I)> | 25.26 | 67.28 | 1.56 |
Completeness [%] | 98.4 | 98.2 | 87.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |