4F0V

Crystal structure of type effector Tse1 from Pseudomonas aeruginousa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	38.265, 61.723, 74.094
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.522 - 1.600
R-factor	0.1566
Rwork	0.156
R-free	0.17210
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.957
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.7.3_928)
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.085	0.373
Number of reflections	23887
<I/σ(I)>	67.5	11.3
Completeness [%]	100.0	100
Redundancy	13.8	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1M Succinic acid pH 7.0, 15% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K