4F0M
UNACTIVATED RUBISCO with MAGNESIUM AND A WATER MOLECULE BOUND
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-17 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 136.325, 136.325, 121.524 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.230 - 2.250 |
| R-factor | 0.16288 |
| Rwork | 0.159 |
| R-free | 0.23416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rub |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.774 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.230 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.101 | 0.751 |
| Number of reflections | 27220 | |
| <I/σ(I)> | 12.1 | 1.3 |
| Completeness [%] | 98.9 | 93.1 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | 0.05 M potassium phosphate, 26% Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, temperature 298.0K |
