4F03
Crystal structure of the glutathione transferase GTE1 from Phanerochaete chrysosporium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.989, 88.383, 157.007 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.539 - 1.800 |
| R-factor | 0.1926 |
| Rwork | 0.192 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.025 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.230 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.619 |
| Number of reflections | 111495 | |
| <I/σ(I)> | 22.2 | 3.6 |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 277 | 30% PEG 8000, 200mM sodium acetate, 100 mM sodium cacodylate, pH 6.5, MICROBATCH, temperature 277K |
