4EZW
Crystal structure of the substrate binding domain of E.coli DnaK in complex with the designer peptide NRLLLTG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99188 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 157.124, 78.026, 89.482 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.700 - 1.800 |
| R-factor | 0.19361 |
| Rwork | 0.193 |
| R-free | 0.22628 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.106 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.044 | 0.455 |
| Number of reflections | 102300 | |
| Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 292 | 2.4 M ammonium sulfate, 0.1 M citric acid pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
