4EZP
Crystal structure of the substrate binding domain of E.coli DnaK in complex with A3-APO(residues 1 to 20)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.681, 160.924, 44.792 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.650 |
| R-factor | 0.2042 |
| Rwork | 0.203 |
| R-free | 0.24212 |
| Structure solution method | PDB ENTRY 3DPO |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.952 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC (with 3DPO) |
| Refinement software | REFMAC (with 3DPO) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.045 | 0.479 |
| Number of reflections | 65834 | |
| Completeness [%] | 96.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292 | 2.4 M ammonium sulfate, 0.1 M sodium acetate pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
