4EZF

The Crystal Structure of a Human MitoNEET mutant with an Ala inserted between Asp 67 and Lys 68

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-06
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	39.626, 55.356, 58.924
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.350 - 1.190
R-factor	0.1647
Rwork	0.165
R-free	0.16710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 2QH7
RMSD bond length	0.009
RMSD bond angle	1.668
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			1.230
High resolution limit [Å]	1.190	2.560	1.190
Rmerge	0.050	0.028	0.791
Number of reflections	41819	4489	3545
<I/σ(I)>	20.28	55.62	1.98
Completeness [%]	98.7	99.4	89.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1