4EY0
Structure of tandem SH2 domains from PLCgamma1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.900, 59.200, 79.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.420 - 2.800 |
| R-factor | 0.1992 |
| Rwork | 0.197 |
| R-free | 0.24190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.420 | 47.470 | 2.950 |
| High resolution limit [Å] | 2.795 | 8.840 | 2.800 |
| Rmerge | 0.021 | 0.762 | |
| Number of reflections | 22779 | ||
| <I/σ(I)> | 17.1 | 1.1 | |
| Completeness [%] | 92.1 | 99.4 | 92.1 |
| Redundancy | 2 | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 18% PEG 8000, 0.1M HEPES.Na, 0.2M Calcium Acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
