4EXT

Structure of polymerase-interacting domain of human Rev1 in complex with translesional synthesis polymerase zeta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Detector technology	CCD
Detector	ADSC QUANTUM 270
Spacegroup name	P 21 21 21
Unit cell lengths	48.699, 71.523, 84.053
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.900
Rwork	0.224
R-free	0.28510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.171
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.042	0.027	0.297
Number of reflections	22498
<I/σ(I)>	28.1
Completeness [%]	96.0	98.7	80.9
Redundancy	5.2	6.4	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	293	0.1M HEPES-Na (pH 7.5), 0.1M NH4-citrate tribasic (pH 7.0), 19-21% PEG 3350, EVAPORATION, temperature 293K