4EXJ
Crystal structure of glutathione s-transferase like protein lelg_03239 (target efi-501752) from lodderomyces elongisporus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-09 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.675, 87.185, 55.346 |
| Unit cell angles | 90.00, 115.17, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.640 |
| R-factor | 0.18482 |
| Rwork | 0.184 |
| R-free | 0.22451 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ay8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.115 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.670 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.770 | |
| Number of reflections | 57493 | |
| <I/σ(I)> | 12.8 | 1.5 |
| Completeness [%] | 98.9 | 90.1 |
| Redundancy | 4.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 0.1M LITHIUM SULFATE, 0.1M BIS-TRIS 25% PEG3350, PH 5.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
