4EWP
Crystal structure of FabH from Micrococcus luteus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.140, 85.026, 96.916 |
| Unit cell angles | 69.82, 71.37, 85.66 |
Refinement procedure
| Resolution | 49.797 - 2.198 |
| R-factor | 0.1709 |
| Rwork | 0.169 |
| R-free | 0.20980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.071 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_718)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.800 |
| High resolution limit [Å] | 2.198 |
| Completeness [%] | 96.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 0.1 M Bis-Tris propane pH 9.0, 10% PEG 200, 18% PEG 8,000, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
