4EWC
Crystal Structure of the Infectious Salmon Anemia Virus Nucleoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-01 |
Detector | NOIR-1 |
Wavelength(s) | 0.97895 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 112.983, 112.983, 103.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.978 - 2.700 |
R-factor | 0.2679 |
Rwork | 0.268 |
R-free | 0.27060 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.133 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.120 | 0.458 |
Number of reflections | 18972 | |
<I/σ(I)> | 9.8 | 2.9 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 5.4 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4 | 283 | 20% PEG6000, 0.8M Lithium Chloride, 0.1M Sodium Citrate, pH 4.0, VAPOR DIFFUSION, temperature 283K |