4EVA
Crystal Structure of Mouse Catenin beta-59 in 5.6M urea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.271, 102.719, 187.559 |
| Unit cell angles | 90.00, 90.14, 90.00 |
Refinement procedure
| Resolution | 28.609 - 2.003 |
| R-factor | 0.2015 |
| Rwork | 0.200 |
| R-free | 0.22770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.703 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.600 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.061 | |
| Number of reflections | 70231 | |
| <I/σ(I)> | 27.5 | 3.04 |
| Completeness [%] | 85.9 | 75.7 |
| Redundancy | 6.6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 1.6~3.2 M Urea, 200mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
