4ETQ
Vaccinia virus D8L IMV envelope protein in complex with Fab of murine IgG2a LA5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.021, 91.156, 103.774 |
| Unit cell angles | 90.00, 107.32, 90.00 |
Refinement procedure
| Resolution | 36.200 - 2.100 |
| R-factor | 0.2026 |
| Rwork | 0.202 |
| R-free | 0.25190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1NSN AND 3JXF |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.123 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.086 | 0.047 | 0.459 |
| Number of reflections | 77173 | ||
| <I/σ(I)> | 10.6 | ||
| Completeness [%] | 97.8 | 95.4 | 93.2 |
| Redundancy | 3.1 | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M potassium thiocyanate, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
