4ESY
Crystal Structure of the CBS Domain of CBS Domain Containing Membrane Protein from Sphaerobacter thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.090, 74.118, 50.981 |
| Unit cell angles | 90.00, 99.13, 90.00 |
Refinement procedure
| Resolution | 38.845 - 2.011 |
| R-factor | 0.184 |
| Rwork | 0.180 |
| R-free | 0.22600 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.419 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_920)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 24825 | |
| <I/σ(I)> | 10.4 | 2.1 |
| Completeness [%] | 99.0 | 89.6 |
| Redundancy | 5.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M MES pH 7.5, 10 %(w/v) PEG 8000, 8 %(v/v) Ethylene Glycol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
