4ESU
Crystal structure of rabbit ryanodine receptor 1 mutant S2776M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 32.154, 56.193, 113.775 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.890 - 1.590 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.22930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ert |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.097 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 56.890 | 1.640 | |
| High resolution limit [Å] | 1.590 | 7.130 | 1.590 |
| Rmerge | 0.065 | 0.027 | 0.590 |
| Number of reflections | 27184 | 346 | 1836 |
| <I/σ(I)> | 12.47 | 33.84 | 2.09 |
| Completeness [%] | 95.6 | 87.6 | 89.2 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 25% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K |
