4ESK
Crystal structure of a strand-swapped dimer of Mouse Leukocyte-associated immunoglobulin-like receptor 1 (NYSGRC-006047)IG-like domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0750 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.141, 73.121, 90.609 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.510 - 1.762 |
| R-factor | 0.1853 |
| Rwork | 0.184 |
| R-free | 0.21610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ety |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.354 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.510 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 40723 | |
| <I/σ(I)> | 59.3 | 6.7 |
| Completeness [%] | 97.5 | 85.8 |
| Redundancy | 14.5 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Sitting Drop Vapor Diffusion | 6 | 298 | Protein (8.5mg/ml in 20 mM Trizma Base pH 8.2, 100 mM NaCl; Reservoir (1.0 M K/Na tartrate MES pH 6.0 - Wizard I & II #98); Cryoprotection (30% glycerol in Reservior solution), Sitting Drop Vapor Diffusion, temperature 298K |
