4ESG
X-ray structure of WDR5-MLL1 Win motif peptide binary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.917 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.606, 80.154, 83.517 |
| Unit cell angles | 90.00, 89.96, 90.00 |
Refinement procedure
| Resolution | 40.080 - 1.700 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.222 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.111 | 0.059 | 0.495 |
| Number of reflections | 55756 | ||
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 99.4 | 99.9 | 93.8 |
| Redundancy | 6.7 | 6.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.2 | 294 | PEG3350, Ammonium Sulfate, HEPES, pH 7.2, hanging drop, temperature 294K |
