4ESG
X-ray structure of WDR5-MLL1 Win motif peptide binary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Detector technology | CCD |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.917 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.606, 80.154, 83.517 |
Unit cell angles | 90.00, 89.96, 90.00 |
Refinement procedure
Resolution | 40.080 - 1.700 |
R-factor | 0.164 |
Rwork | 0.162 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.222 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
Rmerge | 0.111 | 0.059 | 0.495 |
Number of reflections | 55756 | ||
<I/σ(I)> | 11.6 | ||
Completeness [%] | 99.4 | 99.9 | 93.8 |
Redundancy | 6.7 | 6.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7.2 | 294 | PEG3350, Ammonium Sulfate, HEPES, pH 7.2, hanging drop, temperature 294K |