4ERQ
X-ray structure of WDR5-MLL2 Win motif peptide binary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.917 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.780, 80.790, 88.120 |
| Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
| Resolution | 29.775 - 1.906 |
| R-factor | 0.1671 |
| Rwork | 0.165 |
| R-free | 0.19970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.137 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 76235 |
| <I/σ(I)> | 9 |
| Completeness [%] | 1.0 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7.3 | 294 | PEG3350, Ammonium Sulfate, HEPES, pH 7.3, hanging drop, temperature 294K |
