4EQF
Trip8b-1a#206-567 interacting with the carboxy-terminal seven residues of HCN2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9999 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 72.320, 72.320, 146.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.808 - 3.000 |
R-factor | 0.2293 |
Rwork | 0.227 |
R-free | 0.28070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fch |
RMSD bond length | 0.007 |
RMSD bond angle | 1.135 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX (1.7.1_743) |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.910 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.092 | 0.990 |
Number of reflections | 8364 | |
<I/σ(I)> | 15.1 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 91mM MES, 91mM triSodium citrate, 3.63M NaCL, VAPOR DIFFUSION, SITTING DROP, temperature 298K |