4EP9

CRYSTAL STRUCTURE OF RAT CARNITINE PALMITOYLTRANSFERASE 2 IN COMPLEX WITH CoA-site inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	80
Detector technology	CCD
Collection date	2005-12-13
Detector	MARRESEARCH
Wavelength(s)	0.979550
Spacegroup name	P 43 21 2
Unit cell lengths	67.500, 67.500, 308.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.700 - 2.030
R-factor	0.18165
Rwork	0.178
R-free	0.24287
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2deb
RMSD bond length	0.013
RMSD bond angle	1.333
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	2.150
High resolution limit [Å]	2.030	2.030
Completeness [%]	98.4	91.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Hampton Index #76 0.2 M lithium sulfate monohydrate, 0.1 M HEPES pH 7.5, 25 % (w/v) polyethylene glycol 3,350 , VAPOR DIFFUSION, HANGING DROP, temperature 293K