4EOZ

Crystal structure of the SPOP BTB domain complexed with the Cul3 N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	213.166, 76.944, 85.834
Unit cell angles	90.00, 108.34, 90.00

Refinement procedure

Resolution	19.610 - 2.400
R-factor	0.2137
Rwork	0.211
R-free	0.26270
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.449
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.610	2.486
High resolution limit [Å]	2.400	2.400
Number of reflections	49937
<I/σ(I)>	20.4	2.2
Completeness [%]	95.6	74
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.025M Hepes pH 6.5, 0.1M MgCl2, 13% PEG 2000, 11% 2,4-Methyl-2-Pentanediol, VAPOR DIFFUSION, HANGING DROP, temperature 298K