4EOX
X-ray Structure of Polypeptide Deformylase Bound to a Acylprolinamide inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2009-11-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 43 |
Unit cell lengths | 49.681, 49.681, 92.748 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.681 - 1.783 |
R-factor | 0.1845 |
Rwork | 0.182 |
R-free | 0.22960 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.745 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: dev_934)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 92.750 |
High resolution limit [Å] | 1.780 |
Rmerge | 0.054 |
Number of reflections | 21449 |
<I/σ(I)> | 17.8 |
Completeness [%] | 99.9 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M HEPES, 2 M Ammonium Sulfate, 2% PEG400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |