4EOX
X-ray Structure of Polypeptide Deformylase Bound to a Acylprolinamide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-11-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 43 |
| Unit cell lengths | 49.681, 49.681, 92.748 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.681 - 1.783 |
| R-factor | 0.1845 |
| Rwork | 0.182 |
| R-free | 0.22960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.745 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_934)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 92.750 |
| High resolution limit [Å] | 1.780 |
| Rmerge | 0.054 |
| Number of reflections | 21449 |
| <I/σ(I)> | 17.8 |
| Completeness [%] | 99.9 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M HEPES, 2 M Ammonium Sulfate, 2% PEG400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
