4EOU
Crystal structure of E. coli dihydrodipicolinate synthase with pyruvate and succinic semi-aldehyde bound in active site
Replaces: 3UBSExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-07-16 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.172, 121.172, 109.732 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.100 - 2.300 |
| R-factor | 0.14646 |
| Rwork | 0.143 |
| R-free | 0.19779 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yxc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.940 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.100 | 2.359 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.103 | 0.325 |
| Number of reflections | 35072 | |
| <I/σ(I)> | 4.8 | 4.7 |
| Completeness [%] | 89.1 | 90.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10 | 277 | 6% w/v N-octyl-R-glucopyranoside, 1.8 M potassium phosphate dibasic, pH 10.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
