4EOT

Crystal structure of the MafA homodimer bound to the consensus MARE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-04-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 31 2 1
Unit cell lengths	116.100, 116.100, 85.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.972 - 2.855
R-factor	0.2227
Rwork	0.221
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a5t
RMSD bond length	0.002
RMSD bond angle	0.696
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	4.000	3.000
High resolution limit [Å]	2.855	3.600	2.855
Number of reflections	15793
<I/σ(I)>	22.55		3.03
Completeness [%]	97.5	100	85.1
Redundancy	10.8		9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	291	1.2 M ammonium sulfate, 100 mM sodium acetate, pH 4.5, one week of growth, VAPOR DIFFUSION, SITTING DROP, temperature 291K