4ENJ

Crystal structure of S. pombe Atl1 in complex with damaged DNA containing O6-hydroxyethylguanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1158
Spacegroup name	P 61 2 2
Unit cell lengths	59.774, 59.774, 232.724
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.674 - 3.099
R-factor	0.2311
Rwork	0.225
R-free	0.28280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.593
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.099	3.099
Number of reflections	4929
Completeness [%]	97.1	77.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	288	15% mPEG 2000, 0.2M imidazole-malate, 30% xylose, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 288K