4EMH
Crystal structure of SpLsm4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 185.121, 124.470, 131.567 |
Unit cell angles | 90.00, 135.07, 90.00 |
Refinement procedure
Resolution | 92.559 - 2.200 |
R-factor | 0.2376 |
Rwork | 0.237 |
R-free | 0.25270 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.308 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.910 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 105873 | |
Completeness [%] | 99.3 | 91.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.1M MES, pH6.5, 0.1M NaCl, 12% polyethylene glycol (PEG) 4000, VAPOR DIFFUSION, HANGING DROP, temperature 288K |