4EKJ

Crystal structure of a monomeric beta-xylosidase from Caulobacter crescentus CB15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.46
Spacegroup name	P 43 21 2
Unit cell lengths	71.185, 71.185, 226.703
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.700 - 2.500
R-factor	0.1913
Rwork	0.188
R-free	0.24816
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w91
RMSD bond length	0.009
RMSD bond angle	1.277
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.700	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	20621
Completeness [%]	97.5	97.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	ammonium sulphate cobalt chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K