4EJ3

Crystal structure of a CRISPR associated protein from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-05
Detector	ADSC QUANTUM 210
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	93.776, 47.829, 129.178
Unit cell angles	90.00, 97.34, 90.00

Refinement procedure

Resolution	49.860 - 2.520
R-factor	0.22079
Rwork	0.218
R-free	0.27693
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.151
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	38561
<I/σ(I)>		36.2
Completeness [%]	99.9	100
Redundancy		3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2.5M sodium formate, 0.1M Mes, 5% DMSO, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2.5M sodium formate, 0.1M Mes, 5% DMSO, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K