4EIG

CA1698 camel antibody fragment in complex with DHFR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 65
Unit cell lengths	72.270, 72.270, 140.371
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.475 - 2.500
R-factor	0.1744
Rwork	0.172
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.090
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.475	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	14192
Completeness [%]	95.3	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20% PEG w/v 1500, 0.1M Tris, 0.1M Ammonium Sulphate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K